Hardware

Terms

You may use hardware of this site that is linked or described on this page for any personal, commercial or educational purpose, including installing it on as many different computers as you wish. You may also copy, distribute, modify, and/or sell it under the terms of the GNU General Public License (GPL) as published by the Free Software Foundation – either version 2 of the License, or (at your option) any later version. In granting you this right, the GPL requires that the source code you distribute is itself available under the GPL. If you have questions, please contact us.

 

Below is a list of open source hardware developed at the Murphy Lab.

 

Diesel2P Enclosure

 

“[…] We introduce an open-source system with Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging (Diesel2p). Combining optical design, adaptive optics, and temporal multiplexing, the system offers sub cellular resolution over a large field-of-view of ~25 mm2, encompassing distances up to 7 mm, with independent scan engines.” (Yu et al., 2021, para. 1).

Links

OSF Page – includes CAD files and additional resources.

DOI to research article.

Yu, C. H., Stirman, J. N., Yu, Y., Hira, R., & Smith, S. L. (2021). Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry. Nature communications, 12(1), 6639. https://doi.org/10.1038/s41467-021-26736-4

 

Standardized 3D Test Object for Multi-Camera Calibration

 

“Accurate capture of animal behaviour and posture requires the use of multiple cameras to reconstruct 3D representations. […] The proposed 3D printed object has no bias to a particular dimension and is designed to minimize occlusions. The use of the calibrated test object provided an estimate of 3D reconstruction accuracy.” (Hu et al., 2023, para. 1).”

Links

OSF Page – includes CAD files and additional resources.

DOI to research article.

Hao Hu, Roark Zhang, Tony Fong, Helge Rhodin, Timothy H. Murphy, “Standardized 3D test object for multi-camera calibration during animal pose capture,” Neurophoton. 10(4) 046602 (7 November 2023)  https://doi.org/10.1117/1.NPh.10.4.046602

  

Mouse Holder

 

Structure designed to hold mouse in place inside tube, via a headset which connects to the clamps, as shown on the right.

Links

Tubes (Amazon)

CAD Files: includes .stl and .SLDPRT files for tube holding base + clamp pieces.

DOI to research article.

Michelson NJ, Bolaños F, Bolaños LA, Balbi M, LeDue JM, Murphy TH. Meso-Py: Dual Brain Cortical Calcium Imaging in Mice during Head-Fixed Social Stimulus Presentation. eNeuro. 2023 Dec 18;10(12):ENEURO.0096-23.2023. doi: 10.1523/ENEURO.0096-23.2023. PMID: 38053472; PMCID: PMC10731520.

Dual Brain Imaging

 

“[…] a cost-effective, compact foot-print, and open-source Raspberry Pi-based widefield imaging system. The compact nature allows the system to be used for close-proximity dual-brain cortical mesoscale functional-imaging to simultaneously observe activity in two head-fixed animals in a staged social touch-like interaction.” (Michelson et al., 2023, para. 1).

Links

OSF Page – Includes CAD files for components, build instructions, data files, & other resources.

DOI to research article.

Michelson NJ, Bolaños F, Bolaños LA, Balbi M, LeDue JM, Murphy TH. Meso-Py: Dual Brain Cortical Calcium Imaging in Mice during Head-Fixed Social Stimulus Presentation. eNeuro. 2023 Dec 18;10(12):ENEURO.0096-23.2023. doi: 10.1523/ENEURO.0096-23.2023. PMID: 38053472; PMCID: PMC10731520.

 

Tandem Lens Adapters

 

Connecting piece for lens to create new focal lengths

The Adapter Parameters can be adjusted to suit specific lenses.

  • The diameter refers to the inner diameter of the lenses.
  • Pitch is usually 0.5 or 0.75 mm.

Designed by Joel Tsuchitori for the Murphy Lab.

Links

Lens Adapters can be created parametrically using this Onshape Model

 

Glass Slide Cutting Jigs

 

Mold for cutting cover slips.

The Glass Slide Cutting Jig is parameterized by a variable table which determines the depth of the first and second cuts.

  • Made to cut 8mm diameter glass slides
  • When 3D printing: fine print settings should be used.

Designed by Joel Tsushitori for the Murphy Lab

Links

Onshape Model

 

Automatic Mouse Weighing in Double Cage

 

Automatic mouse weighing in group homecages, with Raspberry Pi micro-computers

Links

Python Code assembly instructions

Blender 3D (zip file)

3D Printer files

Python Code

 

Mouse Automated Homecages

 

“[…] improved automated open-source methodology for head-fixed mesoscale cortical imaging and/or behavioral training of home cage mice using Raspberry Pi-based hardware. Staged partial and probabilistic restraint allows mice to adjust to self-initiated headfixation over 3 weeks’ time with ~50% participation rate.” (Murphy et al., 2020).

Links

Python Code and 3D Printer Files

Research Article

 

Mouse Motor Task Homecages

 

Links

Individualized tracking of self-directed motor learning in group-housed mice performing a skilled lever positioning task in the home cage

An Automated Home-Cage System to Assess Learning and Performance of a Skilled Motor Task in a Mouse Model of Huntington’s Disease

 

Software and hardware for automated mouse tapered beam test

This device can also be used for any application where the touch of a paw or tongue needs to be tracked.

Links:

3D printer files

Python code

Ardesch DJ, Balbi M, Murphy TH. Automated touch sensing in the mouse tapered beam test using Raspberry Pi. J Neurosci Methods. 2017;291:221-226. doi:10.1016/j.jneumeth.2017.08.030