Disease Stroke/Psychiatric/Huntington’s

Wang Y, Sepers MD, Xiao D, Raymond LA, and Murphy TH 2023 Water-Reaching Platform for Longitudinal Assessment of Cortical Activity and Fine Motor Coordination Defects in a Huntington Disease Mouse Model.  eNeuro  doi: 10.1523/ENEURO.0452-22.2022

Wang Y, LeDue JM, Murphy TH. 2022 Multiscale imaging informs translational mouse modeling of neurological disease. Neuron. 2022 Nov 16;110(22):3688-3710. doi: 10.1016/j.neuron.2022.09.006 https://doi.org/10.1101/2021.07.18.452688

Balbi M., Xiao D, Vega MJ, Hu H, Vanni M, Bernier L-P, LeDue J, MacVicar BA, Murphy TH (2021) Gamma frequency activation of inhibitory neurons in the acute phase after stroke attenuates vascular and behavioural dysfunction. Cell Reports 34, 108696


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Cait J., Hughes M.R., Chan AW, Osterhof S., Scott R.W., Canals Hernaez, Cait A., Vogel AW, Bernatchez P., Underhill M.T., Granville D., Murphy T.H., Roskelley, C., McNagny  K.M. (2019) Podocalyxin is required for maintaining blood-brain barrier function during acute inflammation.  Proc Natl Acad Sci U S A. 2019 Mar 5;116(10):4518-4527. doi: 10.1073/pnas.1814766116. Epub 2019 Feb 20.

Balbi, M., Vanni, M.P., Vega, M.J., Silasi, G., Sekino, Y., Boyd, J.D., LeDue, J.M. & Murphy, T.H. (2018) Longitudinal monitoring of mesoscopic cortical activity in a mouse model of microinfarcts reveals dissociations with behavioral and motor function. J Cereb Blood Flow Metab, 271678X18763428.

Langdon, K.D., Cordova, C.A., Granter-Button, S., Boyd, J.D., Peeling, J., Murphy, T.H. & Corbett, D. (2017) Executive dysfunction and blockage of brain microvessels in a rat model of vascular cognitive impairment. J Cereb Blood Flow Metab, 271678X17739219.

Balbi, M., Vanni, M.P., Silasi, G., Sekino, Y., Bolanos, L., LeDue, J.M. & Murphy, T.H. (2017) Targeted ischemic stroke induction and mesoscopic imaging assessment of blood flow and ischemic depolarization in awake mice. Neurophotonics, 4, 035001.

McGirr, A., LeDue, J., Chan, A.W., Xie, Y. & Murphy, T.H. (2017) Cortical functional hyperconnectivity in a mouse model of depression and selective network effects of ketamine. Brain, 140, 2210-2225.

Corbett, D., Carmichael, S.T., Murphy, T.H., Jones, T.A., Schwab, M.E., Jolkkonen, J., Clarkson, A.N., Dancause, N., Weiloch, T., Johansen-Berg, H., Nilsson, M., McCullough, L.D. & Joy, M.T. (2017a) Enhancing the Alignment of the Preclinical and Clinical Stroke Recovery Research Pipeline: Consensus-Based Core Recommendations From the Stroke Recovery and Rehabilitation Roundtable Translational Working Group. Neurorehabil Neural Repair, 31, 699-707.

Demuth, H.-U., Dijkhuizen, RM., Farr, TD., Gelderblom, M., Horsburgh, K., Iadecola, C., McLeod, DD., Michalski, D., Murphy, TH., Orbe, J., Otte, W., Petzold, GC., Plesnila, N., Reiser, G., Reymann, KG., Rueger, MA., Saur, D., Savitz, SI., Schilling, S., Spratt, NJ., Turner, RJ., Vemuganti, R., Vivien, D., Yepes, M., Zille, M., and Botze, J. (2017) Recent progress in translational research on neurovascular and neurodegenerative disorders. Proceedings from the 9th International Symposium on Neuroprotection and Neurorepair. 35:87-103.

Hefendehl, J., LeDue, J., Ko, R., Murphy, T., and MacVicar, B. (2016). Mapping synaptic glutamate transporter dysfunction in vivo to regions surrounding Aβ-plaques by iGluSnFR Two-Photon imaging. Nature Comm. 11:127-134.

Connor SA, Ammendrup-Johnsen I, Chan AW, Kishimoto Y, Murayama C, Kurihara N, Tada A, Ge Y, Lu H, Yan R, LeDue JM, Matsumoto H, Kiyonari H, Kirino Y, Matsuzaki F, Suzuki T, Murphy TH, Wang YT, Yamamoto T, Craig AM. (2016) Altered Cortical Dynamics and Cognitive Function upon Haploinsufficiency of the Autism-Linked Excitatory Synaptic Suppressor MDGA2. Neuron 2016 Sep 7;91(5):1052-68. doi: 10.1016/j.neuron.2016.08.016.

Parsons MP, Vanni MP, Woodard CL, Kang R, Murphy TH, Raymond LA (2016) Real-time imaging of glutamate clearance reveals normal striatal uptake in Huntington disease mouse models. Nat Commun 7:11251.

Hilton BJ, Anenberg E, Harrison TC, Boyd JD, Murphy TH, Tetzlaff W (2016) Re-Establishment of Cortical Motor Output Maps and Spontaneous Functional Recovery via Spared Dorsolaterally Projecting Corticospinal Neurons after Dorsal Column Spinal Cord Injury in Adult Mice. J Neurosci 36:4080-4092.

Murphy TH (2015) Two-Photon Imaging of Neuronal Structural Plasticity in Mice during and after Ischemia. Cold Spring Harb Protoc 2015:548-557.

Silasi G, She J, Boyd JD, Xue S, Murphy TH (2015) A mouse model of small-vessel disease that produces brain-wide-identified microocclusions and regionally selective neuronal injury. J Cereb Blood Flow Metab 35:734-738. Supplementary material.

Lim, D.H., LeDue, J.M., Mohajerani, M.H. and Murphy T.H. (2014) Optogenetic mapping after stroke reveals network-wide scaling of functional connections and heterogeneous recovery of the peri-infarct. J. of Neurosci. 34(49):16455-16466.

Xie, Y., Chen, S., and Murphy, T.H. (2014) Prolonged deficits in parvalbumin interneuron synaptic transmission despite recovery of dendritic structure and excitability in somatosensory cortex following global ischemia. J. of Neurosci. 34(45):14890-900.

Silasi, G., and Murphy, T.H. (2014b). Stroke and the Connectome:How Connectivity Guides Therapeutic Intervention. Neuron 83, 1354-1368.

Silasi, G., and Murphy, T.H. (2014a). Removing the brakes on post-stroke plasticity drives recovery from the intact hemisphere and spinal cord. Brain 137, 648-650.

Anenberg, E., Arstikaitis, P., Niitsu, Y., Harrison, T.C., Boyd, J.D., Hilton, B.J., Tetzlaff, W., and Murphy, T.H. (2014). Ministrokes in channelrhodopsin-2 transgenic mice reveal widespread deficits in motor output despite maintenance of cortical neuronal excitability. J Neurosci 34: 1094-1104.

Harrison, T.C., Boyd J.D., Silasi G., Murphy T.H. (2013) Displacement of sensory maps and disorganization of motor cortex after targeted stroke in mice. Stroke. Aug;44(8):2300-6.

Xie Y, Chen S, Anenberg E, Murphy TH. (2013) Resistance of optogenetically evoked motor function to global ischemia and reperfusion in mouse in vivo. J Cereb Blood Flow Metab 33:1148-1152.

Xie, Y., Chen, S., and Murphy, T. (2012). Dendritic spines and pre-synaptic boutons are stable despite local deep hypothermic challenge and re-warming in vivo. PLoS One 7, e36305.

Tran S, Chen S, Liu RR, Xie Y, Murphy TH (2012). Moderate or deep local hypothermia does not prevent the onset of ischemia-induced dendritic damage. J Cereb Blood Flow Metab 32:437–442.

Mohajerani MH, Aminoltejari K, Murphy TH. (2011) Targeted mini-strokes produce changes in interhemispheric sensory signal processing that are indicative of disinhibition within minutes. Proc Natl Acad Sci U S A 2011 May 31;108(22):E183-91.

Chen S, Tran S, Sigler A, Murphy TH (2011) Automated and quantitative image analysis of ischemic dendritic blebbing using in vivo 2-photon microscopy data. J Neurosci Methods 195:222-231.

Sigler, A. and Murphy T.H. (2010) In vivo two-photon imaging of fine structure in the rodent brain: before, during and after stroke. Stroke 41 (10 Suppl): S117-123.

Milnerwood AJ, Gladding CM, Pouladi MA, Kaufman AM, Hines RM, Boyd JD, Ko RW, Vasuta OC, Graham RK, Hayden MR, Murphy TH, and Raymond LA (2010) Early increase in extrasynaptic NMDA receptor signaling and expression contributes to phenotype onset in Huntington’s disease mice. Neuron 65:178-90.

Liu RR, and Murphy TH (2009) Reversible cyclosporine A sensitive mitochondrial depolarization occurs within minutes of stroke onset in mouse somatosensory cortex in vivo. A two-photon imaging study. J Biol Chem 284:36109-36117. Supplemental Movies.

Murphy TH, and Corbett D (2009) Plasticity during stroke recovery: from synapse to behaviour. Nat Rev Neurosc 10:861-872.

Brown CE, Boyd JD, and Murphy TH (2009) Longitudinal in vivo imaging reveals balanced and branch specific dendritic remodelling of mature cortical pyramidal neurons after stroke. J Cereb Blood Flow Metabol, doi:10.1038/jcbfm.2009.241.

Winship IR and Murphy TH (2009) Re-mapping the somatosensory cortex brain after stroke: insight from imaging the synapse to network. The Neuroscientist 15:507-524.

Sigler A, Mohajerani MH, and Murphy TH (2009) Imaging rapid redistribution of sensory-evoked depolarization through existing cortical pathways after targeted stroke in mice. Proc Natl Acad Sci U S A. 106: 11759-64.

Brown CE, Aminoltejari K, Erb H, Winship I, and Murphy TH (2009) In vivo voltage sensitive dye imaging in adult mice reveals that somatosensory maps lost to stroke are replaced over weeks by new structural and functional circuits with prolonged modes of activation within both the peri-infarct zone and distant sites. J. Neurosci. 2009 29:1719-1734.  Supplemental Movies. See our cover photo above. 

Li P and Murphy TH (2008) Two-photon imaging during prolonged middle cerebral artery occlusion in mice reveals recovery of dendritic structure following reperfusion. J Neurosci 28:11970-11979.

Winship IR and Murphy TH (2008) In vivo calcium imaging reveals functional rewiring of single somatosensory neurons after stroke. J. Neurosci. 28:6592-6606. * See comments in Research Highlights, Neuroscience: Rewiring the brain. Nature 454, 140; doi:10.1038/454140b.

Murphy TH, Li P, Betts K, and Liu R (2008) Two-photon imaging of stroke onset in vivo reveals that NMDA-receptor independent ischemic depolarization is the major cause of rapid reversible damage to dendrites and spines. J Neurosci 28:1756-1772.

Sigler A, Goroshkov A, and Murphy TH (2008) Hardware and methodology for targeting single brain arterioles for photothrombotic stroke on an upright microscope. J Neurosci Methods 170:35-44. Supplemental Movie.

Brown CE and Murphy TH (2008) Livin’ on the edge: imaging dendritic spine turnover in the peri-infarct zone during ischemic stroke and recovery. Neuroscientist 14: 139-146.

Brown CE, Wong C, and Murphy TH (2008) Rapid morphologic plasticity of peri-infarct dendritic spines after focal ischemic stroke. Stroke 39:1286-1291.

Brown CE, Li P, Boyd JD, Delaney KR, and Murphy TH (2007) Extensive turnover of dendritic spines and vascular remodeling in cortical tissues recovering from stroke. J Neurosci 27:4101-4109.

Zhang S and Murphy TH (2007) Imaging the impact of cortical microcirculation on synaptic structure and sensory-evoked hemodynamic responses in vivo. PLoS Biol 5:e119.

Enright LE, Zhang S, and Murphy TH (2007) Fine mapping of the spatial relationship between acute ischemia and dendritic structure indicates selective vulnerability of layer V neuron dendritic tufts within single neurons in vivo. J Cereb Blood Flow Metab 27:1185-1200. See our cover photo above. 

Zhang S, Boyd J, Delaney K, and Murphy TH (2005) Rapid reversible changes in dendritic spine structure in vivo gated by the degree of ischemia. J Neurosci 25:5333-5338.