IO and VSD Signal Processor


Plugin for ImageJ for analysis of Intrinsic Optical Signal (IOS) or Voltage Sensitive Dye (VSD) fluorescence signal in imaging data. Written by Albrecht Sigler.


Updated version: January 6, 2012







Installation file

To install, copy the file into the plugin folder and restart ImageJ. Detailed information about handling plugins can be found at the Plugins website of ImageJ. Requires ImageJ version 1.31v or later, whereas updating to newer ImageJ versions is recommended for performance.
For further analysis of the resulting (ΔF/F0) image stacks we suggest using the Dynamic Z Profiler plugin (former name “Intensity v Time Monitor”; source code: Z

Source code

Open Source; feel free to modify and to distribute according to the rules of the GNU General Public License.

Program Description

PDF file with detailed program description and user guide.










Harrison TC, Sigler A, Murphy TH. Simple and cost-effective hardware and software for functional brain mapping using Intrinsic Optical Signal imaging. J Neurosci Methods. 2009;182(2):211-218. doi:10.1016/j.jneumeth.2009.06.021